Antiviral responses of human Leydig cells to mumps virus infection or poly I:C stimulation
Identifieur interne : 001349 ( Main/Exploration ); précédent : 001348; suivant : 001350Antiviral responses of human Leydig cells to mumps virus infection or poly I:C stimulation
Auteurs : A. Le Tortorec [France] ; H. Denis [France] ; A-P. Satie [France] ; J-J. Patard [France] ; A. Ruffault [France] ; B. Jgou [France] ; N. Dejucq-Rainsford [France]Source :
- Human Reproduction [ 0268-1161 ] ; 2008-09.
Abstract
BACKGROUND The immuno-privileged status of the testis is essential to the maintenance of its functions, and innate immunity is likely to play a key role in limiting harmful viral infections, as demonstrated in the rat. In men mumps virus infects Leydig cells and has deleterious effects on testosterone production and spermatogenesis. The aim of this study was to test whether mumps virus infection of isolated human Leydig cells was associated with an inhibition of their innate antiviral defences. METHODS Leydig cell production of mRNA and protein for interferons (IFNs) and of three antiviral proteins25 oligoadenylate synthetase (25OAS), double-stranded RNA-activated protein kinase (PKR) and MxAwas investigated, in the absence or presence of mumps virus or viral stimuli including poly I:C, a mimetic of RNA viruses replication product. RESULTS Stimulated or not, human Leydig cells appeared unable to produce routinely detectable IFNs , and . Although the level of PKR remained unchanged after stimulation, the expression of 25OAS and MxA was enhanced following either mumps virus or poly I:C exposure (P < 0.05 versus control). CONCLUSIONS Overall, our results demonstrate that mumps virus replication in human Leydig cells is not associated with a specific inhibition of IFNs or 25OAS, MxA and PKR production and that these cells display relatively weak endogenous antiviral abilities, as opposed to their rat counterparts.
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DOI: 10.1093/humrep/den207
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Patard</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Service d'Urologie, Centre Hospitalier Universitaire Rgional Pontchaillou, Rennes</wicri:regionArea><placeName><settlement type="city">Rennes</settlement></placeName></affiliation></author><author><name sortKey="Ruffault, A" sort="Ruffault, A" uniqKey="Ruffault A" first="A." last="Ruffault">A. Ruffault</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Unit de Rtrovirologie, Centre Hospitalier Universitaire Rgional Pontchaillou, Rennes</wicri:regionArea><placeName><settlement type="city">Rennes</settlement></placeName></affiliation></author><author><name sortKey="Jgou, B" sort="Jgou, B" uniqKey="Jgou B" first="B." last="Jgou">B. Jgou</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Inserm, U625, Rennes</wicri:regionArea><placeName><settlement type="city">Rennes</settlement></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Univ Rennes I, Campus de Beaulieu, IFR-140, Groupe d'Etude de la Reproduction chez l'Homme et les Mammifres, Rennes, F-35042</wicri:regionArea><placeName><region type="region" nuts="2">Région Bretagne</region></placeName></affiliation></author><author><name sortKey="Dejucq Rainsford, N" sort="Dejucq Rainsford, N" uniqKey="Dejucq Rainsford N" first="N." last="Dejucq-Rainsford">N. 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In men mumps virus infects Leydig cells and has deleterious effects on testosterone production and spermatogenesis. The aim of this study was to test whether mumps virus infection of isolated human Leydig cells was associated with an inhibition of their innate antiviral defences. METHODS Leydig cell production of mRNA and protein for interferons (IFNs) and of three antiviral proteins25 oligoadenylate synthetase (25OAS), double-stranded RNA-activated protein kinase (PKR) and MxAwas investigated, in the absence or presence of mumps virus or viral stimuli including poly I:C, a mimetic of RNA viruses replication product. RESULTS Stimulated or not, human Leydig cells appeared unable to produce routinely detectable IFNs , and . Although the level of PKR remained unchanged after stimulation, the expression of 25OAS and MxA was enhanced following either mumps virus or poly I:C exposure (P < 0.05 versus control). CONCLUSIONS Overall, our results demonstrate that mumps virus replication in human Leydig cells is not associated with a specific inhibition of IFNs or 25OAS, MxA and PKR production and that these cells display relatively weak endogenous antiviral abilities, as opposed to their rat counterparts.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Région Bretagne</li></region><settlement><li>Rennes</li></settlement></list><tree><country name="France"><noRegion><name sortKey="Le Tortorec, A" sort="Le Tortorec, A" uniqKey="Le Tortorec A" first="A." last="Le Tortorec">A. Le Tortorec</name></noRegion><name sortKey="Dejucq Rainsford, N" sort="Dejucq Rainsford, N" uniqKey="Dejucq Rainsford N" first="N." last="Dejucq-Rainsford">N. Dejucq-Rainsford</name><name sortKey="Dejucq Rainsford, N" sort="Dejucq Rainsford, N" uniqKey="Dejucq Rainsford N" first="N." last="Dejucq-Rainsford">N. Dejucq-Rainsford</name><name sortKey="Dejucq Rainsford, N" sort="Dejucq Rainsford, N" uniqKey="Dejucq Rainsford N" first="N." last="Dejucq-Rainsford">N. Dejucq-Rainsford</name><name sortKey="Denis, H" sort="Denis, H" uniqKey="Denis H" first="H." last="Denis">H. Denis</name><name sortKey="Denis, H" sort="Denis, H" uniqKey="Denis H" first="H." last="Denis">H. Denis</name><name sortKey="Jgou, B" sort="Jgou, B" uniqKey="Jgou B" first="B." last="Jgou">B. Jgou</name><name sortKey="Jgou, B" sort="Jgou, B" uniqKey="Jgou B" first="B." last="Jgou">B. Jgou</name><name sortKey="Le Tortorec, A" sort="Le Tortorec, A" uniqKey="Le Tortorec A" first="A." last="Le Tortorec">A. Le Tortorec</name><name sortKey="Patard, J J" sort="Patard, J J" uniqKey="Patard J" first="J-J." last="Patard">J-J. Patard</name><name sortKey="Ruffault, A" sort="Ruffault, A" uniqKey="Ruffault A" first="A." last="Ruffault">A. Ruffault</name><name sortKey="Satie, A P" sort="Satie, A P" uniqKey="Satie A" first="A-P." last="Satie">A-P. Satie</name><name sortKey="Satie, A P" sort="Satie, A P" uniqKey="Satie A" first="A-P." last="Satie">A-P. Satie</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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